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Sains Malaysiana 53(4)(2024): 781-794
http://doi.org/10.17576/jsm-2024-5304-05
(Modulasi Sel Stem Mesenkima Sumsum Tulang Manusia (BMMSC) oleh Nigella sativa dan Madu Trigona: Suatu Kajian in vitro)
1Department of Medical
Sciences I, Faculty of Medicine and Health Sciences, Universiti Sains Islam Malaysia, 71800 Nilai, Negeri Sembilan, Malaysia
2Department of Medical
Sciences II, Faculty of Medicine and Health Sciences, Universiti Sains Islam Malaysia, 71800 Nilai, Negeri Sembilan, Malaysia
Received: 27
April 2023/Accepted: 27 February 2024
Abstract
Maintenance of bone marrow mesenchymal stem cells
(BMMSC) numbers is crucial for tissue repair and regeneration since adult stem
cells are often limited in number, expansion capacity and lifespan. It is
believed that certain types of foods are beneficial in the preservation and
stimulation of stem cells throughout life. Black seeds and stingless bee honey
are among the widely investigated functional food with general health promoting
benefit. We aim to explore the
proliferative, anti-apoptosis and anti-senescence effect of Nigella sativa and Trigona honey
on BMMSC. Cell proliferation was evaluated using MTT assay. We performed flow cytometric analysis to
verify stem cell surface markers while quantitative PCR was done to measure the
relative expression of target genes. Results
showed favourable concentration dependent enhancement of cell
proliferation by 1 to 10 ug/mL Nigella sativa and 0.097% to 0.195% Trigona honey.
Observation on BMMSC’s morphology and surface markers expression revealed no
alteration on BMMSC stemness properties. In addition,
gene expression analysis supported that there was a significant (P<0.05)
increase in proliferation related gene, β-Catenin, and lower expression of
apoptotic- and senescence-related gene, BAX and p21, respectively. These
preliminary findings suggest a promising effect of Nigella sativaand Trigona honey on stem cell proliferation.
Keywords: Apoptosis; black
seed; bone-marrow mesenchymal stem cells; proliferation; senescence; stingless
bee honey
Abstrak
Pengekalan bilangan sel stem mesenkima sumsum tulang
(BMMSC) adalah penting untuk pembaikan dan penjanaan semula tisu kerana sel
stem dewasa selalunya terhad dalam bilangan, kapasiti pengembangan dan jangka
hayat. Jenis makanan tertentu adalah dipercayai bermanfaat dalam pemeliharaan
dan ransangan sel stem sepanjang hayat. Habbatus sauda dan madu lebah kelulut
adalah antara makanan bermanfaat terhadap kesihatan yang dikaji secara meluas.
Penyelidikan ini bertujuan untuk meneroka kesan proliferatif, anti-apoptosis
dan anti-penuaan Nigella sativa dan madu Trigona terhadap BMMSC.
Proliferasi sel telah dinilai menggunakan ujian MTT. Kami menggunakan analisis
aliran sitometriks untuk mengesahkan penanda permukaan sel stem
manakala PCR kuantitif dilakukan untuk mengukur pengekspresan relatif gen
sasaran. Keputusan menunjukkan proliferasi sel yang menggalakkan dalam
kepekatan sebanyak 1 hingga 10 ug/mL Nigella sativa dan 0.096 hingga 0.195% madu Trigona. Pemerhatian pada morfologi dan ekspresi penanda permukaan BMMSC menunjukkan tiada perubahan pada sifat BMMSC. Tambahan pula, analisispengekspresan gen menyokong bahawa terdapat peningkatan yang ketara (P<0.05) dalam gen proliferatif, β-Catenin manakala gen apoptosis dan gen penuaan, BAX dan p21,
masing-masing menunjukkan ekspresi yang lebih rendah. Penemuan awal ini
mencadangkan kesan memberangsangkan Nigella sativa dan madu Trigona terhadap proliferasi sel stem.
Kata kunci: Apoptosis; Habbatus sauda; madu lebah
kelulut; penuaan; proliferatif; sel stem mesenkima sumsum tulang
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*Corresponding author; email: nurfariha@usim.edu.my
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